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KMID : 0613820030130050692
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Journal of Life Science
2003 Volume.13 No. 5 p.692 ~ p.698
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Effects of Pine Needle Ethyl Acetate Fraction on Membrane Fluidity and Oxidative Stress in Brain Membranes of Rats
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Choi Jin-Ho
Kim Dae-Ik
Baek Seung-Jin
Park Si-Hyang
Kim Nam-Ju
Cho Weon-Ki
Kim Koon-Ja
Kim Chang-Mok
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Abstract
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This study was designed to investigate the effects of ethyl acetate (EtOAc) fraction of pine (Pinus densiflora Sieb et Zucc) needle on membrane fluidity (MF), basal and induced oxygen radical (BOR and IOR), lipid peroxide (LPO) and oxidized protein (OP) as a oxidative stress, and lipofuscin (LF) in brain membranes of Sprague-Dawley (SD) rats. Male SD rats were fed basic diets (control) and experimental diets (EtOAc-25, EtOAc-50 and EtOAc-100) for 45 days. MF was significantly increased (about 10%) in mitochondria of EtOAc-100 group. BOR and IOR formations in mitochondria were significantly inhibited (about 9～10% and 17～24%, respectively) in EtOAc-50 and EtOAc-100 groups, while BOR and IOR formations in microsomes were significantly inhibited (about 12～17% and 12～16%, respectively) in EtOAc-50 and EtOAc-100 groups compared with control group. LPO levels in mitochondria and microsomes were significantly inhibited (about 9～12% and 12～19%, respectively) in EtOAc-50 and EtOAc-100 groups, whereas significant difference between OP or LF levels and control group in these membranes could not be obtained. These results suggest that administrations of ethyl acetate fraction of pine needle may play an effective role in an attenuating an oxidative stress and in increasing membrane fluidity.
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KEYWORD
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Pine (Pinus densiflora Sieb et Zucc), Lipid peroxide (LPO), Oxidized protein, Carbonyl group, Membrane fluidity(MF), Lipofuscin (LF).
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